甜樱桃花粉萌发率测定条件的优化研究

Deciduous Fruits（2018）

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Abstract

在常规花粉萌发培养液成分的基础上,进行优化培养液中有利于花粉萌发的各因子配比,对花粉萌发率短时高效测定的培养条件进行优化选择,研究了蔗糖浓度、硼酸浓度及培养液pH值对甜樱桃花粉萌发的效果.结果表明,在培养液中硼酸浓度0.01％和pH值6.0不变,蔗糖浓度20％时,甜樱桃花粉萌发率最高;培养液中蔗糖浓度10％和pH值6.0不变,硼酸浓度0.1％时,甜樱桃花粉萌发率最高;培养液中蔗糖浓度10％和硼酸浓度0.01％不变,pH值6.5时甜樱桃花粉萌发率最高.结合双凹片法培养4小时,可较省时有效地测定甜樱桃花粉萌发率.

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