甜樱桃花粉萌发率测定条件的优化研究
Deciduous Fruits(2018)
Abstract
在常规花粉萌发培养液成分的基础上,进行优化培养液中有利于花粉萌发的各因子配比,对花粉萌发率短时高效测定的培养条件进行优化选择,研究了蔗糖浓度、硼酸浓度及培养液pH值对甜樱桃花粉萌发的效果.结果表明,在培养液中硼酸浓度0.01%和pH值6.0不变,蔗糖浓度20%时,甜樱桃花粉萌发率最高;培养液中蔗糖浓度10%和pH值6.0不变,硼酸浓度0.1%时,甜樱桃花粉萌发率最高;培养液中蔗糖浓度10%和硼酸浓度0.01%不变,pH值6.5时甜樱桃花粉萌发率最高.结合双凹片法培养4小时,可较省时有效地测定甜樱桃花粉萌发率.
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