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蝴蝶兰种胚快繁技术研究

王梅,陈火星,吴在生,陈丽,李铭

AGRICULTURE ENGINEERING TECHNOLOGY(GREENHOUSE HORTICULTORE)（2010）

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Abstract
本试验以盆栽蝴蝶兰'火鸟'为材料,采用常规杂交方法获取果荚.果荚采后进行消毒处理,采用不同培养基对种子胚进行无菌播种试验,筛选最佳种子胚播种培养基.结果表明:果荚采摘最佳时间为授粉后120 d,采用75%酒精30 s和0.1%升汞6 min的消毒处理污染率最低,花宝1号2.0 g/L+蛋白胨2.0 g/L+肌醇0.1 g/L+磷酸三钙0.1 g/L+香蕉汁100 g/L+蔗糖20 g/L+琼脂7.0 g/L是蝴蝶兰无菌播种的最佳培养基.
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