奶牛关节炎病原菌的分离与鉴定
China Animal Husbandry & Veterinary Medicine(2016)
Abstract
为避免奶牛关节炎对牛场造成巨大经济损失,采集患病奶牛关节积液,应用胰蛋白胨肉汤琼脂分离培养病原菌,随后通过形态学方法、生化试验和PCR扩增16S rRNA基因片段及TA克隆测序对其进行鉴定.结果显示,该菌在血琼脂上产生明显的β溶血;光镜下可见该菌个体为单个或短链状排列的革兰氏阳性球菌;该菌七叶苷、麦芽糖、蔗糖、葡萄糖、过氧化氢酶试验均为阳性,对头孢唑啉、恩诺沙星、环丙沙星、氨苄西林、链霉素、氯霉素、新霉素、头孢吡肟8种抗生素敏感,对四环素、红霉素、强力霉素、阿米卡星4种抗生素耐药;该菌与金黄色葡萄球菌核苷酸同源性为91%,但与肉葡萄球菌核苷酸同源性为92%.依据该菌株16S rRNA基因片段测序结果,并结合生物学特性分析,初步将分离菌株鉴定为葡萄球菌属的细菌.本试验结果可为该牛场确诊和防治关节炎提供必要的试验依据.
MoreKey words
dairy cow arthritis,Staphylococcus spp,16S rRNA,biological characteristics
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