棘孢木霉葡聚糖酶基因Glu1的克隆及原核表达
Pratacultural Science（2018）
Abstract
从棘孢木霉(Trichoderma asperellum)ACCC30536中克隆获得一个葡聚糖酶基因Glu1,其cDNA全长984 bp,编码327个氨基酸.该葡聚糖酶属于Glyco-hydro 12家族,推测为p-1,4-葡聚糖酶,与深绿木霉IMI 206040的Glyco-hydro-12家族蛋白(XP_013940397.1)有91％相似性,且亲缘关系较近.运用qRT-PCR技术检测9种诱导条件下棘孢木霉Glu1基因的表达水平,结果表明,Glu1基因能参与棘孢木霉对山新杨(Populus davidiana×P.alba var.pyramidlis)或杨树病原菌的识别.构建原核表达载体并获得重组蛋白rGlu1.酶活特性分析结果表明,该酶最适pH为4.5,最适温度为45℃,且酶活性随诱导时间延长逐渐升高,在5h达到稳定.
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