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兔抗O型口蹄疫VLPs酶标抗体的制备及初步应用

Jia-hui HE,Man-yuan BAI,Hui-chen GUO,Shi-qi SUN,Xiao-li ZHANG,Xiao-jun MA

Progress in Veterinary Medicine（2019）

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Abstract
为制备兔抗O型口蹄疫病毒样颗粒 (VLPs) 的酶标抗体, 进一步建立O型口蹄疫病毒的ELISA检测方法, 通过原核表达、体外纯化和组装O型口蹄疫VLPs, 免疫家兔制备兔抗O型口蹄疫VLPs高免血清, 并采用亲和层析法纯化IgG, 改良过碘酸钠法制备兔抗O型口蹄疫VLPs的酶标抗体 (rabbit anti-type O foot-and-mouth disease IgG-HRP) .结果显示, 兔抗O型口蹄疫VLPs高免血清效价达1:4 096, 纯化后的IgG纯度达90％, 效价达1:512 000.表明获得了高纯度的兔抗O型口蹄疫VLP的酶标抗体, 可用于O型口蹄疫的ELISA检测.
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