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饲用淀粉酶产生菌株的筛选及产酶条件优化

贾国宾,李卓伟,杨国辉,魏丽娟,张沛,王德功,刘冬

China Feed（2015）

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Abstract
本试验以鸡、猪、牛空肠黏膜为菌源,进行产淀粉酶菌株的筛选.通过初筛、复筛得到一株产酶活较高的菌株YZ-ZH03. 通过单因素试验考察碳源、氮源等对菌株产酶活力的影响,并采用正交试验优化发酵工艺. 试验结果表明最佳发酵工艺条件为:葡萄糖1.0%,尿素1.5%,NaCl 0.10%,K2HPO4 0.4%,KH2PO4 0.1%,初始pH 6.0,250 mL三角瓶装液量50 mL,接种量4%,摇瓶转速200 r/min,发酵温度37℃,培养时间30 h. 酶活力与未优化前相比提高了33.66%.
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