禽白血病病毒K亚群RT-LAMP检测方法的建立

Chinese Veterinary Science(2018)

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Abstract
根据GenBank数据库中禽白血病病毒(ALV)K亚群特异性的gp85基因序列,设计了特异性检测ALV K亚群(ALV-K)的RT-LAMP引物.对内、外引物浓度组合和反应温度进行优化,建立了ALV-K的RT-LAMP检测方法.特异性试验结果显示,本方法只对ALV-K进行特异性扩增,而对禽白血病的其他亚群以及NDV、REV、AIV、MDV、IBV等常见禽病病原未见扩增.敏感性试验表明,本方法最低能够检测到3.4×104 copies/μL的质粒.
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Key words
virus,rt-lamp
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