布鲁菌疫苗株Rev.1全基因序列的测定与分析

Huan-yu YU,Zhi-dan ZHANG,Jian-qi LIU,Dan-tong ZHAO,Li-xia ZHAO,Guo-ying LIU,Qing-qing SONG,Guo-hua WANG,Xiang-chen PAN,Dong-dong LIU,Pu-hou TIAN,Shun-li SHI,Ping-yuan GUAN

Animal Husbandry and Feed Science（2020）

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Abstract

为促进布鲁菌病Rev.1疫苗及相关疫苗的研发,该研究提取Rev.1疫苗株核酸,应用PacBio平台进行全基因序列测定与分析.结果表明,Rev.1疫苗株基因组大小约3 299 187 bp,G+C含量为57.2％,组装为染色体1、染色体2两条环状基因组,大小分别为2 121 370、1 177 817 bp,G+C含量分别为57.2％、57.3％.将其Ery、BLS和VirB10基因序列与9株GenBank上发表的布鲁菌参考菌株的Ery、BLS和VirB10基因序列进行比较分析,存在不同程度的差异,同源性为97.4％～100％.

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