HPLC法和LC-MS/MS法测定饲料中黄曲霉毒素B1含量的比较
Animal Husbandry and Feed Science(2019)
Abstract
比较了HPLC光化学衍生化法、HPLC三氟乙酸衍生化法、LC-MS/MS法对饲料中黄曲霉毒素B1(AFB1)的检测结果进行验证和比较,为AFB1实验室检测工作中适宜方法的选择提供参考依据.结果表明:HPLC光化学衍生化法和HPLC三氟乙酸衍生化法均有良好的线性范围和相关系数(R≥0.99997),AFB1的回收率为88.4%~104.6%,精密度均小于5%;LC-MS/MS法相关系数R≥0.99900,AFB1的回收率为85.3%~96.8%,精密度小于3%.提示对于饲料中AFB1的检测,HPLC光化学衍生化法操作简单、灵敏度高、稳定性好,但检测成本较高;HPLC三氟乙酸衍生化法检测成本低,但样品基质干扰大,操作较繁琐;LC-MS/MS法定性准确,但定量准确度稍差,检验成本最高.
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