乳酸片球菌素Ped-A基因表达载体的构建
Feed Review(2015)
Abstract
乳酸片球菌素可以作为防腐剂延长食品的保鲜期,为研究和开发可食用级别的防腐剂,将乳酸片球菌素结构基因Ped-A全长和不合信号肽的序列克隆至乳酸菌表达载体pMG36e和pNZ8048,经酶切鉴定及序列分析,所转化的大肠杆菌DH5α和DHB4中含有插入Ped-A基因全长和不合信号肽的序列的重组质粒.结果表明,成功构建了乳酸片球菌素Ped-A基因的乳酸菌表达载体pMG 36e/Ped-A(全长/片段)、pNZ8048/Ped-A(全长/片段).
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