猪流行性腹泻病毒N蛋白杆状病毒表达与间接ELISA方法建立
Chinese Journal of Veterinary Medicine(2016)
Abstract
为了检测猪群中猪流行性腹泻(PED)抗体水平,用Bac-to-Bac杆状病毒表达系统,构建含有PEDV N基因的重组杆状病毒rBac-PEDV N并在昆虫细胞中获得表达.用纯化的重组N蛋白作为包被抗原,建立了检测PEDV抗体的间接酶联免疫吸附试验(ELISA)方法.与Western Blot的符合率为91.3%,说明该方法适用于PED的临床诊断.
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