一株鸡传染性喉气管炎病毒的分离鉴定
HEBEI XUMU SHOUYI(2019)
Abstract
从山西省大同市某养鸡场采集临床疑似鸡传染性喉气管炎的病鸡喉头气管,接种鸡胚绒毛尿囊膜分离病毒,获得6株分离毒,经PCR鉴定为传染性喉气管炎病毒,血凝试验结果显示3株有血凝性,剩余3株经常见外源病毒PCR检测,结果显示1株无常见外源.以分离毒人工感染SPF鸡,于攻毒后3d出现传染性喉气管炎的典型临床症状,剖检亦可见喉头气管黏膜出血,气管中大量黏液渗出等病理变化,且从发病鸡的喉头内重新分离到鸡传染性喉气管炎病毒.
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