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常规PCR与实时荧光定量PCR(qPCR)技术敏感性比较

郭文涛,徐爱玲,加洛,冯建萍,米宝玉,周奎章,李千,吴海生,栗冬梅

Chinese Qighai Journal of Animal and Veterinary Sciences（2020）

Cited 1|Views28
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Abstract
对70匹采自青海省海南藏族自治州兴海县和贵南县喜马拉雅旱獭体表寄生蚤2科3属3种,包括斧形盖蚤(Callopsylla dolabris)、谢氏山蚤(Oropsyllasilantiewi)、人蚤(Pulex irritans)进行巴尔通体检测,并对11个阳性样品进行常规PCR和实时荧光定量PCR (qPCR)的敏感性比较.通过常规PCR反应产物和qPCR反应产物琼脂糖凝胶电泳分析,发现qPCR检测敏感性高于常规PCR.此项技术的应用将对巴尔通体感染的早期快速诊断、监测和流行病学调查等研究提供有效方法.
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