C57BL/6J小鼠促性腺激素注射时间对诱导超排卵的影响
Chinese Journal of Animal Husbandry and Veterinary Medicine（2015）
Abstract
目的:确定4周龄C57BL/6J小鼠注射促性腺激素的最佳间隔时间,提高超排效果,从而优化C57BL/6J近交系作为供体鼠的生物净化体系.方法:70只4周龄的C57BL/6J雌鼠随机分为5组,腹腔注射10IU的PMSG,10IU的hCG注射间隔时间分别为47h,47.5h,48h,48.5h,49h,比较超排的卵母细胞数和受精率等数据.结果:第1组与第5组的卵母细胞数有极显著差异,其他数据差异不显著.结论:4周龄C57BL/6J小鼠对PMSG应答产生的内源性黄体生成素(LH)释放量不足以引起排卵,因而hCG注射时间无需严格控制在注射PMSG后46～48h内.
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