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乙醇浸泡蜜蜂标本DNA的提取纯化

董霞,史宪伟,汪建明,赵灵芝,陈永久

APICULTURE OF CHINA（2001）

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Abstract
利用RAPD、PCR、RFLP、分子杂交等分子生物学技术研究生物的遗传变异、遗传多态、分子进化等,必须以DNA作为模板,目前所见有关DNA提取纯化的报道均用活体或冰冻蜜蜂,这给样品采集、保存带来不便.本实验以采自云南不同地区以及马来西亚的蜜蜂乙醇(75%)浸泡标本为材料,参照王文(1994年)的蛋白酶K提取法并加以改进,对DNA进行提取纯化.
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