检测猪传染性胃肠炎病毒RT-PCR的建立及应用

栗永华, 郭露露,徐力, 梁瑞婷, 陈小苹,陈金山,郑玉姝

Henan journal of animal husbandry and veterinary medicine(2015)

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Abstract
根据GenBank上猪传染性胃肠炎病毒S基因的保守序列,设计1对引物,建立了检测猪群中的猪传染性胃肠炎病毒的RT-PCR方法.试验结果表明,该RT-PCR方法敏感、特异,适于临床样品检测,为猪传染性胃肠炎病毒的临床诊断和流行病学调查奠定了基础.
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