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骆驼刺源产乳酸的枯草芽孢杆菌分离鉴定和菌株特性研究

Yating LI,Lu YING,Yonghua SU,Hui JIANG

Heilongjiang Animal Science and Veterinary Medicine（2016）

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Abstract
为了解疏叶骆驼刺青贮中乳酸菌的组成,用MRS培养基对骆驼刺青贮中的细菌进行分离和纯化,通过革兰氏染色、细胞形态学观察、生理生化试验和16S rDNA序列分析对分离的单菌落进行菌株特性研究和种属鉴定.结果表明:L14菌株呈杆状,G+,有芽孢;产乳酸,液化明胶,水解淀粉,触酶阳性,温度生长范围15～55℃,酸生长范围pH值为4～7,NaCl生长范围为2％ ～ 10％,可利用多种碳源和氮源;16S rDNA测序结果显示该菌株与ATCC 6051的相似性为98.7％,说明该菌为枯草芽孢杆菌(Bacillus subtilis).
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