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大片形吸虫CatL1蛋白的原核表达及其特异性多克隆抗体的制备

Leming WANG,Linjing HOU,Guoshun RAO,Xuefang MEI,Wei SHI,Yurui WANG,Zhengjiao WU,Dongying WANG,Weiyu ZHANG

Heilongjiang Animal Science and Veterinary Medicine（2020）

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Abstract
为了制备大片形吸虫诊断抗体,试验采用原核表达制备大片形吸虫组织蛋白酶L1重组蛋白(rFgCatL1),用该蛋白免疫Balb/c小鼠制备多克隆抗体,通过间接酶联免疫吸附法(ELISA)检测抗体效价,Western-blot法检测多克隆抗体的特异性.结果 表明:CatL1基因大小为981 bp,蛋白质分子质量为39.0 ku左右,与预期分子质量大小一致.制备的小鼠多克隆抗体血清效价达1∶128 000,能特异性识别大片形吸虫排泄-分泌物(ESP)抗原.说明制备的rFgCatL1多克隆抗体可作为良好的诊断抗体用于研制快速诊断试剂盒.
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