血鹦鹉鱼Mitfa基因表达qPCR 分析的引物设计与评估
Hebei Fisheries(2018)
Abstract
MITF,即小眼畸形相关转录因子(microphthalmia-associated transcription factor),其在黑色素细胞的发育和分化中具有核心的调控作用.为了解色素相关基因和体色之间的关系,应用分析血鹦鹉的表达谱,采用实时荧光定量PCR(qPCR)技术技术,从血鹦鹉(Amphilophus)鱼鳍与鱼皮中提取RNA,通过Primer 5软件设计扩增血鹦鹉Mitfa基因片段的引物并进行qPCR评估,为血鹦鹉Mitfa基因表达的qPCR分析奠定了基础.
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