荷花转录组测序及花青素苷合成相关基因表达分析

Ya'nan Meng,Lin Zhang,Shaoqiang Liu,Fangfang Shi,Yiping Liu,Dezheng Kong

Journal of Southwest Forestry University（2018）

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Abstract

以荷花不同花色品种 '青玉'和 '白洋淀红莲'为研究对象,运用高通量测序技术,对松蕾期的花瓣进行转录组测序,并对2个测序文库进行差异表达及实时荧光定量PCR分析.结果表明:共获得1142条差异表达基因.筛选出与花青素苷合成相关的关键基因ANS、CHS、DFR、UF3GT. 4个基因在两种荷花花瓣不同时期中的表达量存在显著差异,在松蕾期或初花期的表达量最高.

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