丙酸睾酮对小鼠睾丸NT-3和NT-4表达的影响
Journal of Jilin Agricultural University（2016）
Abstract
以30日龄雄性小鼠为研究对象,用丙酸睾酮分别处理小鼠6,12,24 h后收集睾丸,提取睾丸总RNA和总蛋白,通过实时荧光定量和Western-Blot方法探究了雄激素对睾丸中神经营养因子NT-3及NT-4表达的影响.荧光定量结果表明:丙酸睾酮处理小鼠,12h组和24 h组NT-3及NT-4的表达显著升高,其他各组间无显著差异.Western-Blot结果与荧光定量结果一致.说明丙酸睾酮能够调节小鼠睾丸中NT-3和NT-4的表达.
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