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一株氯嘧磺隆高效降解菌分离鉴定及降解条件优化

Chunyan LI,Huiming CAO,Xuejiao AN,Luming WEN,Wanjun LIU,Hailian ZANG,Ning HOU

Journal of Northeast Agricultural University（2016）

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Abstract
采用富集培养方法从氯嘧磺隆生产单位排污口处污泥中分离得到一株能降解氯嘧磺隆细菌,命名为D310-5.通过对该菌株形态特征观察,生理生化特性和16S rDNA序列分析,将菌株D310-5鉴定为肠杆菌属(Enterobacter sp.).采用响应面分析方法探究底物浓度、温度、pH和培养时间对菌株D310-5降解氯嘧磺隆影响,优化菌株D310-5对氯嘧磺隆降解条件.结果表明,菌株D310-5最佳降解条件为底物浓度101.57 mg· L-1,温度30.25℃,pH 6.63,培养时间5d.在最佳条件下,菌株D310-5对氯嘧磺隆降解率为87.57％.
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