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革胡子鲶MHC基因表达qPCR分析的引物设计与评估

Zhuan-zhuan LI,Yan WANG,Jin-wei GAO,Xiao-mei WANG,Cheng-xun CHEN, Tao LI,Bao-lin MO

Journal of Tianjin Agricultural College（2016）

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Abstract
为应用实时荧光定量PCR(qPCR)技术分析革胡子鲶主要组织相容性复合体(major histocompatibility complex,MHC)基因的表达谱,本研究利用Primer5软件设计扩增革胡子鲶MHC基因片段的引物并进行qPCR评估.结果显示:引物MHCF1-MHCR3在qPCR扩增时,融解曲线为单一尖锐峰;标准曲线分析显示:引物的扩增效率为99.3％,R2值为0.998.上述结果说明该对引物具有良好的扩增特异性和扩增效率,满足了qPCR扩增的要求.本研究为革胡子鲶MHC Ⅰ基因表达的qPCR分析奠定了基础.
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