玉米瘤黑粉菌SG200效应蛋白PEP1表达及生物信息学分析
Journal of Hunan Agricultural University(Natural Sciences)（2020）
Abstract
运用生物信息学手段分析pep1基因结构,并根据GenBank中玉米瘤黑粉菌pep1 DNA序列设计引物,从玉米瘤黑粉菌SG200的基因组中扩增得到了pep1基因全长,构建了pET-28a-pep1重组表达质粒,选用大肠埃希菌BL21(DE3)作为宿主菌,以1 mmol/L IPTG诱导表达.SDS-PAGE检测结果表明,诱导表达产物大小与理论值(20800)一致,说明pET-28a-pep1能够在大肠埃希菌BL21(DE3)中高效表达.
MoreAI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined