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禽白血病病毒p27抗原的原核表达及其结合性多肽的筛选

Xiangbo JI,Liheng ZHENG,Xinfeng LI, Tianxing CAO,Zhongwei CHEN, Chunxia ZHANG

MODERN ANIMAL HUSBANDRY（2019）

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Abstract
为获取与禽白血病病毒(Avian leukemia virus,ALV) p27抗原高亲和力结合的特异性多肽,原核表达并制备了高纯度的ALV p27蛋白,以纯化的ALV p27蛋白包被ELISA板,用M13噬菌体随机进行筛选.经过4轮淘选,ELISA鉴定噬菌体单克隆抗体与ALV p27蛋白的亲和力,筛选出4个与ALV p27蛋白具有高亲和力的噬菌体单克隆抗体进行DNA测序,并推导出多肽的氨基酸序列.
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