1株拮抗链格孢的芽孢杆菌的筛选鉴定和抑菌效果
Jiangsu Agricultural Sciences（2019）
Abstract
以链格孢(Alternaria spp.)为靶标菌从农田土壤中分离到1株芽孢杆菌.通过形态学特征、生理生化分析及16S rDNA序列分析等对菌株进行鉴定.结果表明,菌株LKYLW-1为萎缩芽孢杆菌(Bacillus atrophaeus,GenBank登录号为MF375905);对链格孢的抑菌带宽为15.8 mm;采用菌丝生长抑制法、孢子萌发法测定LKYLW-1的抑菌作用,发现菌株LKYLW-1代谢产物对链格孢丝有致畸作用;菌株LKYLW-1发酵液对孢子萌发抑制率为96.60％;EC50为6.93 mL/L;饱和度为25％;硫酸铵获得的抑菌物质对链格孢的抑菌活性较高,抑菌圈直径达42.01 mm.
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