野生泸定百合鳞片的组织培养技术
Guizhou Agricultural Sciences(2017)
Abstract
为野生泸定百合资源的保护及开发应用提供依据,以其鳞片为外植体,观察其不定芽的诱导、增殖以及生根培养情况.结果表明:野生泸定百合鳞片用0.1%升汞消毒15 min,效果较好;MS+6-BA1.0 mg/L+NAA0.1 mg/L为野生泸定百合鳞片最佳不定芽诱导培养基,平均每鳞片分化芽数达5.4个;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L为鳞片最佳增殖培养基,增殖系数达7.66;1/2 MS+IBA 1.0 mg/L为鳞片最佳生根培养基,生根率达95.2%,平均根数5.3条,平均根长3.9 cm.
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