谷子白发病菌卵孢子重寄生真菌的分离与鉴定

Yuting YAN, Yongzhi XU, Zhulong HE,Junming GAO

Journal of Shanxi Agricultural Sciences（2019）

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Abstract

为了从谷子白发病菌卵孢子上分离筛选出对谷子白发病具有生防潜力的重寄生真菌,通过石英砂振荡去除卵孢子表面附着物,利用孢悬液稀释点样和尖端菌丝分离法对重寄生真菌进行分离纯化,采用形态学与真菌rDNA-ITS序列分析对分离出的重寄生真菌进行鉴定.结果表明,谷子白发病菌卵孢子上重寄生r有层出镰孢菌(Fusarium proliferatum)、拟轮枝镰孢菌(Fusarium verticillioides)和半裸镰孢菌(Fusarium incarnatum)3种镰孢属真菌.

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