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宁薯10号茎尖离体培养及种薯生产

Cong-fa LIN,Xiang-hua PAN,Lin-lin WENG,Min ZHUO,Ai-qing ZHONG

Guangdong Agricultural Sciences（2013）

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Abstract
为了保持宁薯10号甘薯品种的优良种性,延长种薯的田间应用年限,运用茎尖分生组织培养,指示植物检测方法获得脱毒苗.结果表明,以MS+6-BA 1.0mg/L+NAA 0.1mg/L为宁薯10号茎尖初代培养基,成苗率达94.4％；以6-BA 0.2mg/L+NAA 0.05mg/L为茎切段继代增殖培养基,繁殖系数达2.54倍；宁薯10号脱毒苗产量比对照原品种提高16.5％,达到极显著水平,可大面积推广应用.
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