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血鹦鹉TYR基因表达qPCR分析的引物设计与评估

Xueliang SUN,Weiwei GAO,Hongyue SHI,Zhenzhen FANG,Jian LIANG,Yanbin JI,Chengxun CHEN, Tingying HU

Tianjin Agricultural Sciences（2018）

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Abstract
为了解色素相关基因和体色之间的关系,应用实时荧光定量PCR(qPCR)技术分析血鹦鹉的酪氨酸酶(tyrosinase,TYR)基因的表达谱,通过Primer 5软件设计扩增血鹦鹉TYR基因片段的引物并进行qPCR评估研究.结果显示,引物TYR2F-TYR2R在qPCR扩增时,融解曲线为单一尖锐峰;标准曲线分析显示,引物的扩增效率为103.3％,R2值为0.994.上述结果说明该对引物具有良好的扩增特异性和扩增效率,满足了qPCR扩增的要求,该研究结果可为血鹦鹉TYR基因表达的qPCR分析奠定基础.
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