枯草芽胞杆菌GB519发酵液菌体数量和芽胞率检测方法的比较

Feng ZHU,Jichun WANG,Chengli TIAN, Yuanyuan XIANG, Fuhao ZHANG,Shanyan QI,Dongyuan WANG,Zhaoyuan JIANG,Xiaomei LIU,Li LI,Jinping REN,Zhongyu DOU

Journal of Northeast Agricultural Sciences（2019）

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Abstract

为了提高枯草芽胞杆菌GB519发酵液菌体数量和芽胞率的统计效率,本研究采用平板涂布法和平板倾注法进行活菌计数,利用加热除菌法、结晶紫染色法和孔雀绿染色法开展芽胞率检测效率的比较研究.结果表明:平板涂布法与倾注法的菌体计数数量差异不大,误差率相对一致.枯草芽胞杆菌GB519利用结晶紫染色法与孔雀绿染色法检测芽胞率差异不显著.确定了枯草芽胞杆菌GB519加热除菌法菌体致死温度为80℃、时间为15 min,其芽胞量为131.9×108 CFU/mL,芽胞率为50.1％,与孔雀绿染色法(54.8％)差异不显著,与结晶紫染色法芽胞率(58.7％)差异显著.本研究将为类似的芽胞检测研究提供方法和数据参考.

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