解淀粉芽孢杆菌15-1-1的摇瓶发酵条件优化

Journal of Jilin Agricultural Sciences(2015)

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Abstract
探讨解淀粉芽孢杆菌15-1-1菌株液体发酵条件,为提高其活性次级代谢产物的产量提供支持.通过单因素试验和正交试验,对该菌株的最适发酵培养基成分及发酵条件进行优化,结果表明:15-1-1菌株培养基各组分的最佳配比为:葡萄糖2.0%、蛋白胨0.5%、酵母浸膏1.0%、氯化钠0.5%.最佳发酵培养条件为:初始pH值7.0,培养温度32,培养时间36 h,250 mL三角瓶通气量40 mL,接种量体积分数5%.
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