拉萨大黄组织培养与快速繁殖

Cheng-gang GUO,Hai-lin BI,Wang-wai TANG,Run-guang XUE,Chun-lian XU

Special Wild Economic Animal and Plant Research（2017）

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Abstract

建立拉萨大黄快速繁殖体系,并通过诱导愈伤组织建立拉萨大黄再生体系.以拉萨大黄种子为材料,利用组织培养方法对拉萨大黄进行愈伤组织诱导.结果表明,剥皮种子是拉萨大黄组织培养的最佳外植体;较高浓度的细胞分裂素和相对较低的2,4-D有利于丛生芽的诱导及分化;MS+6-BA 1.0mg/L+KT 1.0mg/L+ZT0.5mg/L+2,4-D0.5mg/L是最佳的初代培养诱导及分化培养基;MS+6-BA2.0mg/L+KT2.0mg/L+ZT 1.0mg/L+2,4-D0.5mg/L为最佳的增殖培养基;1/2MS+NAA 1.0mg/L+2000mg/L活性炭是最佳的生根诱导培养基,生根率98％;炼苗成活率达到90％.

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