枇杷内生木霉P3.9菌株液体发酵条件优化筛选
Hubei Agricultural Sciences(2017)
Abstract
为弄清枇杷内生木霉P3.9菌株最佳发酵条件,以分离自枇杷主干韧皮部的内生木霉P3.9菌株为供试菌株,以菌丝生长量为指标,通过单因素和正交试验,筛选最佳组合液体发酵培养液,碳源、氮源、pH及其接种量.结果表明,最佳培养液为马铃薯浸汁,碳源为D-果糖,氮源为磷酸二氢铵,pH 7.0,接种量为1.5﹪;正交试验表明其菌丝生长的最佳组合培养条件为马铃薯浸汁,在其中添加2﹪D-果糖、0.05﹪磷酸二氢铵、pH 6.0、接种量为1.5﹪.按优化后的培养条件培养,最高菌丝干重可达5.1 mg∕mL.
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