一种高通量水稻DNA提取方法及其在种子纯度检测中的应用

Acta Agriculturae Shanghai(2016)

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Abstract
以杂交节水抗旱稻‘旱优73’和‘旱优3号’及其亲本为材料,采取一种高通量96深孔板提取水稻DNA的方法进行杂交种子纯度鉴定.结果表明:该提取方法获得的DNA纯度高,能满足正常的SSR分析,利用引物RM208和RM336扩增的条带清晰可辨,可对‘旱优73’和‘旱优3号’杂交种子纯度进行准确鉴定.
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