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宁糯芋1号茎尖组培快繁及种芋生产

Bulletin of Agricultural Science and Technology(2019)

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Abstract
为调整福建省芋栽培品种结构,加快优良品种推广应用,以宁糯芋1号田间生长的块茎顶芽或侧芽为外植体,运用茎尖分生组织培养进行了不同TDZ浓度处理试验获得脱毒苗,结果发现,与6-BA相比,TDZ更适于芋茎尖诱导成芽,在MS培养基中附加0.1~0.2 mg/L的TDZ能显著促进芋茎尖的萌动,产生丛生芽.茎尖初代培养基为MS+TDZ 0.2 mg/L时成苗率达78%,继代增殖培养基MS+TDZ 0.3 mg/L繁殖系数达7.88,生根壮苗培养基1/2MS+NAA 0.5 mg/L+活性炭0.2 mg/L.脱毒芋种产量比对照原品种提高29.7%,达到极显著差异水平.
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