诱导前后地鳖血淋巴抗菌活性的研究
Journal of Guangdong University of Technology(2014)
Abstract
采用体内注射法,对中华真地鳖( Eupolyphage sinensis Walker )成虫(雌性)活体分别注射大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌,诱导24、48和72 h后取血淋巴,以未经诱导的血淋巴为对照,用滤纸片法分别测定诱导后血淋巴对此3种菌的抑制作用.选择抗菌活性最强的金黄色葡萄球菌诱导48 h的血淋巴,分析加样量、pH、温度和加热时间等因素对血淋巴抗菌活性的影响.结果表明:随着加样量的增大诱导血淋巴的抑菌效果增强;血淋巴抑菌活性最适pH值为5.0;血淋巴的抗菌活性热敏性较强,在80℃和100℃下短时间内失活.
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