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生物转化制备红参Rh1皂苷组及成分分析

Xiaobing WANG,Chunying LIU,Shang WANG,Hongshan YU,Fengxie JIN

Journal of Dalian Dalian Polytechnic University（2016）

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Abstract
利用TLC和超高效液相色谱-质谱(UPLC-MS)分析已制备的Rh1组异构体,利用HPLC分析其异构体的质量比.Rh1组在TLC图谱中显2个斑点,经UPLC-MS的分析,确认红参皂苷Rh1组含有4种异构体,分别是20(S)-Rh1、20(R)-Rh1、Rk3和Rh4;高效液相色谱检测结果表明红参皂苷Rh1组中4种异构体20(S)-Rh1、20(R)-Rh1、Rk3、Rh4的质量比为25.0∶ 18.8∶15.5∶40.7,4种异构体总质量分数在90％以上.
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Key words
ginsenoside Rh1 group,isomers,HPLC,UPLC-MS
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