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烤烟品种的多重PCR技术标记鉴别

Meng-zhu JIA,Jiu-zhe SUN,Dong-ying SU,Jin-chu YANG,Er-bin WANG,Heng XU,Yong-chao WANG,Jun-ting WANG,Dan-dan MENG,Lin MA

Journal of Zhengzhou University of Light Industry(Natural Science Edition)（2017）

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Abstract
以12份烤烟品种为模板,针对6对SCAR引物进行引物比例和退火温度的条件优化,采用多重PCR技术,建立了12份烤烟品种的多重PCR指纹图谱和鉴别路线.结果表明,同普通PCR相比,多重PCR具有高效、快速、节约时间、成本低的优点,6对SCAR引物稳定性好、条带特异性强,能准确区分12份烤烟品种.
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