谷氨酸棒杆菌argG基因缺失菌株的构建
Journal of Nanjing University of Technology(Natural Science Edition)（2013）
Abstract
构建1株谷氨酸棒杆菌argG基因缺失菌株并观察其对L-瓜氨酸生产的影响.采用crossover PCR方法扩增带有argG基因上下游同源序列的DNA片段,连接至载体pK18mobsacB,构建敲除载体pK18-△argG,转化至C.glutamicum ATCC 13032,利用卡那霉素抗性正筛选和果聚糖蔗糖sacB基因负筛选获得argG基因缺失菌株.发酵结果表明,argG缺失菌株60 h积累2.52 g/LL-瓜氨酸,出发菌C.glutamicum ATCC 13032不能积累L-瓜氨酸.
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