高效液相色谱法测定烟叶中的绿原酸及其异构体
Physical Testing and Chemical Analysis Part B:Chemical Analysis(2019)
Abstract
取经烘干并粉碎的烟叶样品0.1000 g,加入CH 3 OH-H 2 O(1+1)混合液20 mL,超声提取20 min.提取液经0.45μm滤膜过滤,滤液供高效液相色谱法分析.以Diamonsil Plus C18色谱柱为固定相,并用不同比例的(A)甲醇和(B)乙酸-水(1+99)溶液的混合液按设定程序进行梯度洗脱,使样品中的绿原酸及其异构体分离.采用紫外检测器进行检测.结果表明:绿原酸的线性范围在1.01~101.22 mg·L-1之间,其异构体(新绿原酸及隐绿原酸)则同在0.25~25.00 mg·L-1之间,其检出限(3S/N)分别为0.013,0.025,0.028 mg·L-1.按标准加入法进行回收试验,测得回收率在92.0%~104%之间,测定值的相对标准偏差(n=5)在1.1%~4.0%之间.
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