Targeted Delivery Of Sirna And Other Difficult To Transfect Molecules Using Electroporation: Current Status And Future Scope

RNA interference (RNAi) is a natural phenomenon existing in plants, insects, and many mammals involved in gene silencing or the inhibition of gene expression. Small interfering RNAs (siRNAs) could be used as potential tools to implement this process that could lead to treatments in diseases such as cancer. However, the delivery of siRNA to eukaryotic cells is a challenge due to its lower cellular uptake and easy degradation by enzymes in blood, tissues, and cells. Electroporation-based siRNA delivery is an upcoming therapeutic tool to deliver various siRNAs, both in vitro and in vivo. Discussed in this chapter is the review of various successful deliveries of siRNA using electroporation.