60Co γ射线照射对AT和GM细胞增殖能力及O2.-浓度的影响
RADIATION PROTECTION（2005）
Abstract
为探求AT5BIVA细胞辐射敏感性与超氧阴离子自由基O2.-之间的联系,以正常人皮肤成纤维细胞GM0639为对照,用3H-TdR掺入法和细胞色素C还原法分别测定正常及照射条件下细胞增殖能力及其培养介质中O2.-浓度(nmol/106细胞).结果表明,在正常培养条件下,两种细胞的增殖能力和向培养介质中释放O2.-的量均随培养时间的延长而增加.在不同剂量60Co γ射线照射条件下,两种细胞的增殖能力和向培养介质中释放O2.-的量均随剂量增大而减小;两种细胞的增殖能力均与其培养介质中O2.-浓度密切相关,且AT5BIVA细胞的增殖速率大于GM0639细胞.提示AT5BIVA细胞高辐射敏感性与O2.-产生量密切相关.
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