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牛乳中嗜冷菌的PCR快速检测

Jian LOU,Yuke SHEN,Mengyun LIU,Yuxiao JIN, Jianqin YE,Yuru YOU,Jianwei MAO,Shiwang LIU

FAJIAO KEJI TONGXUN（2017）

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Abstract
利用选择性培养基分离牛乳中的嗜冷菌,通过形态学特征观察和生理生化试验鉴定分离株,判断分离株为杆菌属和球菌属.根据菌属基因序列设计PCR引物,提取基因组DNA进行PCR快速检测,通过测序比对确定分离株为嗜冷杆菌和腐生葡萄球菌.
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