酶法转化制备L-瓜氨酸

FINE CHEMICALS(2005)

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Abstract
利用粪链球菌精氨酸脱亚胺酶转化L-精氨酸制备L-瓜氨酸.考察了菌龄、转化温度等多种因素对精氨酸脱亚胺酶活力的影响.酶法制备L-瓜氨酸的最适工艺条件是:菌体发酵时间20 h,转化温度37 ℃,转化液起始pH为6.0,ρ[十六烷基三甲基溴化铵(CTAB)]=0.3 g/L使酶活提高了414%.c(Co2+)=10-3 mol/L使酶活增高约50%,c(Cu2+)=10-2 mol/L或c(Zn2+)=10-2 mol/L使酶活下降约50%.Ca2+、Mg2+和Mn2+对酶活影响较小.
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Key words
enzymatic,conversion,l-citrulline
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