酶催化荧光猝灭法测定痕量葡萄糖

Shun-zhen HE,Fei-fei LI,Yi-song LU,Xi LIN,Xiao-na XU,Sheng-yuan YANG

Applied Chemical Industry（2018）

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Abstract

在pH 7.0的磷酸盐缓冲液中,葡萄糖氧化酶(GOx)催化葡萄糖与氧反应生成H2O2,在H2SO4介质中H2O2与I-发生氧化还原反应生成I2,过量的I-与I2生成I-3,I-3使阳离子荧光剂罗丹明6G位于553 nm波长处的荧光发生猝灭,猝灭程度与葡萄糖浓度相关,据此建立检测葡萄糖的新方法.方法线性范围为2.0×10-7~1.4×10-5mol/L,检出限为6.77 ×10-8mol/L.将该方法用于血糖样品测定,加标回收率为85.0% ~102.5%,操作简便、结果准确可靠.

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