响应面法优化鹿茸胶原蛋白制备抗氧化肽的水解条件
The Chinese Journal of Process Engineering(2020)
Abstract
以鹿茸中下段胶原蛋白为酶解底物,用木瓜蛋白酶酶解制备小分子抗氧化肽,以清除1,1-二苯基-2-苦基肼基(DPPH·)自由基的能力为指标,采用响应面法优化酶水解条件.结果 表明,最优实验条件为时间56 min,酶添加量1.40wt%,pH=5.60,温度60℃.该条件下所得抗氧化肽对DPPH.自由基的清除率为83.09%.用超滤膜、半制备色谱柱和超高效液相色谱-质谱联用仪分级分离获得分子量0.2~0.6 kDa的具有最高抗氧化活性的多肽,其具有与头段类似的保健功效,更易被人体吸收,且易进一步加工和储存.
MoreKey words
antioxidant peptide preparation,enzymatic hydrolysis conditions,velvet antler collagen
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