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载香豆素-6聚乳酸羟基乙酸共聚物纳米粒包封率的测定

Rong SUN,Xiao-yi XU,Wei XU,Yi-hua YANG

Guangzhou Chemical Industry（2018）

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Abstract
利用高效液相色谱(HPLC)法,建立香豆素-6(Cou-6)含量测定方法,采用高速离心法分离游离药物,从而测定Cou-6含量并计算载Cou-6聚乳酸羟基乙酸共聚物(PLGA)纳米粒的包封率.实验所得结果显示Cou-6在1~50 μg·L-1范围内线性关系良好(r=0.9998),平均回收率为100.05% ±2.03%, PLGA纳米粒的包封率为89.65% ±5.23%.根据结果表明该方法简便迅速、准确可靠,为纳米粒的质量评价提供参考.
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