高效液相色谱法测定胡麻卵磷脂中磷脂酰肌醇含量
Shandong Chemical Industry（2018）
Abstract
建立高效液相色谱法测定胡麻卵磷脂中磷脂酰肌醇的方法.使用Agilent 1200高效液相色谱仪和 C18色谱柱(5μm,150mm× 4.6mm),以V(甲醇):V(异丙醇):V(乙腈)=37:61:2为流动相,检测波长为206nm;流速:1.0mL/min;柱温:35℃.定量进样,进样量10μL,以峰面积定量.结果表明:胡麻卵磷脂中磷脂酰肌醇在5.015~25.045 μg/mL浓度范围内线性较好,测定出胡麻卵磷脂中磷脂酰肌醇占5.394%,平均回收率98.58%.该方法可信度较高、分析速度快、操作简便,可为检定胡麻中磷脂酰肌醇含量提供科学参考.
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