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高效液相色谱法测定中药洗发水中侧柏叶槲皮苷的含量

Jing ZHOU,Rong-wen ZHANG,Ri-qiong JIANG,Zhi-qiang WU,Jie-lin REN,Yu-hua WAN

Detergent & Cosmetics（2015）

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Abstract
建立中药洗发水中侧柏叶活性成分含量的HPLC测定方法,色谱条件为以十八烷基键合相硅胶填充剂(150×4.6 mm,5μm);以甲醇-0.01 mol/L磷酸二氢钾溶液-冰醋酸(40:60:1.5)为流动相,流速为1 mL/min;柱温:25℃;进样量为10μL;检测波长为254 nm.以侧柏叶槲皮苷对照品作标准曲线,在0.152～1.52 g/L范围内线性关系良好(r=0.999 5),加样回收率为93.70％,RSD为1.78％.
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